Design of a humanized anti vascular endothelial growth factor nanobody and evaluation of its in vitro function

Authors

  • Fatemeh Kazemi-Lomedasht Venom & Biotherapeutics Molecules Laboratory, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran
  • Mahdi Behdani Venom & Biotherapeutics Molecules Laboratory, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran
  • serge muyldermans Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussels, Belgium
Abstract:

Objective(s): Nanobodies, the single domain antigen binding fragments of heavy chain-only antibodies occurring naturally in camelid sera, are the smallest intact antigen binding entities. Their minimal size assists in reaching otherwise largely inaccessible regions of antigens. However, their camelid origin raises a possible concern of immunogenicity when used for human therapy. Humanization is a promising approach to overcome the problem.   Materials and Methods: Here, we designed a humanized version of previously developed nanobody (anti vascular endothelial growth factor nanobody), evaluated and compared its predicted 3D structure, affinity and biological activity with its original wild type nanobody. Results: Our in silico results revealed an identical 3D structure of the humanized nanobody as compare to original nanobody. In vitro studies also demonstrated that the humanization had no significant visible effect on the nanobody affinity or on its biological activity.  Conclusion: The humanized nanobody could be developed and proposed as a promising lead to target pathologic angiogenesis.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

In vitro combination therapy of pathologic angiogenesis using anti-vascular endothelial growth factor and anti-neuropilin-1 nanobodies

Objective(s): Emergence of resistant tumor cells to the current therapeutics is the main hindrance in cancer treatment. Combination therapy, which mixes two or more drugs, is a way to overcome resistant problems of cancer cells to current treatments.  Nanobodies are promising tools in cancer therapy due to their high affinity as well as high penetration to tumor sites....

full text

P-185: Expression and Function of Vascular Endothelial Growth Factor in Fallopian Tuben Carrying Ectopic Pregnancy

Background: Vascular endothelial growth factor (VEGF) is an angiogenic growth factor that is produced by different tissues, including the endometrium and ovary. VEGF is involved in many processes related to reproductive physiology, including angiogenesis, which is essential for implantation and placentation. It has been suggested that VEGF may be the angiogenic factor responsible for the implan...

full text

Evaluation of Periodontal Disease Effect on the Expression of Vascular Endothelial Growth Factor and Nerve Growth Factor in Dental Pulp

Introduction: Researchers have always been interested in the close and mutual relationship between the pulp and periodontal tissues. Although the effects of pulpal disease on periodontium have been well known, the clear relationship between periodontitis and pulp has not been documented . It seems that bacteria and inflammatory products of periodontitis can affect pulp through accessory canals,...

full text

Diagnostic Value of Serum Levels of Vascular Endothelial Growth Factor as a Marker of Tubal Pregnancy

Background and purpose: Tubal pregnancy is a life threatening condition that not only causes mortality but also reduces fertility by less than 50%. In many patients, the level of βhCG does not reach detection threshold, and for definite diagnosis, long-term follow-up considering βhCG titration and transvaginal ultrasonography are needed which could lead to delay in diagnosis and treatment. The ...

full text

Quantitation of Vascular Endothelial Growth Factor and Interleukin-6 in Different Stages of Breast Cancer

Background: Determination of the impact of angiogenesis on tumor development and progression is essential. This study aimed to determine the serum levels of Vascular endothelial growth factor (VEGF) and Interleukin 6 (IL-6) in breast carcinoma, and to correlate them with tumor size, lymph node involvement, and cancer stage. Methods: Under aseptic precautions 5 ml of venous blood was collecte...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 21  issue 3

pages  260- 266

publication date 2018-03-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023